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anti sp7 antibody  (MedChemExpress)
94
MedChemExpress anti sp7 antibody
D-Bmp2@M accelerates fracture healing in osteoporotic mice. a. Schematic of the osteoporotic fracture treatment procedure: C57BL/6 mice underwent bilateral ovariectomy (OVX) to establish an osteoporosis model, followed by transverse femoral fracture induction and 28 days of treatment. b. Representative X-ray images of the fracture healing process at different time points and Micro-CT 3D reconstruction of femurs on day 28 post-treatment: white arrows (fracture location), red arrows (early callus), blue dotted lines (femur boundary), and yellow dashed lines (callus boundary). Scale bar of x-ray: 5 mm; Scale bar of 3D reconstruction: 1 mm. c. Quantitative analysis of the fracture callus BMD and BV/TV (normal PBS Ctrl group data from PBS group in d–g) (n = 6 per group). d. Representative H&E staining images and representative Masson's trichrome staining images of fracture calluses at 28 days. Scale bar: 50 μm. e. Quantification of the callus area/total bone area ratio and quantification of the new bone area/total bone area ratio (n = 6 per group). f, g. Representative IFHC images of the callus region at 28 days and quantification of the relative fluorescence intensities: (f) The white dotted line represents the boundary between the callus and muscles (M: muscle, C: callus); Runx2 (red), ALP (green), and DAPI (blue). Scale bar: 50 μm; (g) Quantification of the relative fluorescence intensities of Runx2 and ALP (n = 6 per group). h, i. Representative IFHC images of the callus region at 28 days and quantification of the relative fluorescence intensities: (h) The white dotted line represents the boundary between the callus and muscles (M: muscle, C: callus); <t>Sp7</t> (red), ALP (green), and DAPI (blue). Scale bar: 50 μm; (i) Quantification of the relative fluorescence intensities of Sp7 and ALP (n = 6 per group). The data are presented as the means ± standard deviations (SDs). Unpaired Student's t -test was used for two-group comparisons. One-way ANOVA was used for multiple comparisons. Significance levels: ∗ p < 0.05, ∗∗∗∗ p < 0.0001.
Anti Sp7 Antibody, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti sp7  (MedChemExpress)
94
MedChemExpress anti sp7
D-Bmp2@M accelerates fracture healing in osteoporotic mice. a. Schematic of the osteoporotic fracture treatment procedure: C57BL/6 mice underwent bilateral ovariectomy (OVX) to establish an osteoporosis model, followed by transverse femoral fracture induction and 28 days of treatment. b. Representative X-ray images of the fracture healing process at different time points and Micro-CT 3D reconstruction of femurs on day 28 post-treatment: white arrows (fracture location), red arrows (early callus), blue dotted lines (femur boundary), and yellow dashed lines (callus boundary). Scale bar of x-ray: 5 mm; Scale bar of 3D reconstruction: 1 mm. c. Quantitative analysis of the fracture callus BMD and BV/TV (normal PBS Ctrl group data from PBS group in d–g) (n = 6 per group). d. Representative H&E staining images and representative Masson's trichrome staining images of fracture calluses at 28 days. Scale bar: 50 μm. e. Quantification of the callus area/total bone area ratio and quantification of the new bone area/total bone area ratio (n = 6 per group). f, g. Representative IFHC images of the callus region at 28 days and quantification of the relative fluorescence intensities: (f) The white dotted line represents the boundary between the callus and muscles (M: muscle, C: callus); Runx2 (red), ALP (green), and DAPI (blue). Scale bar: 50 μm; (g) Quantification of the relative fluorescence intensities of Runx2 and ALP (n = 6 per group). h, i. Representative IFHC images of the callus region at 28 days and quantification of the relative fluorescence intensities: (h) The white dotted line represents the boundary between the callus and muscles (M: muscle, C: callus); <t>Sp7</t> (red), ALP (green), and DAPI (blue). Scale bar: 50 μm; (i) Quantification of the relative fluorescence intensities of Sp7 and ALP (n = 6 per group). The data are presented as the means ± standard deviations (SDs). Unpaired Student's t -test was used for two-group comparisons. One-way ANOVA was used for multiple comparisons. Significance levels: ∗ p < 0.05, ∗∗∗∗ p < 0.0001.
Anti Sp7, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd3ε  (Novus Biologicals)
95
Novus Biologicals cd3ε
D-Bmp2@M accelerates fracture healing in osteoporotic mice. a. Schematic of the osteoporotic fracture treatment procedure: C57BL/6 mice underwent bilateral ovariectomy (OVX) to establish an osteoporosis model, followed by transverse femoral fracture induction and 28 days of treatment. b. Representative X-ray images of the fracture healing process at different time points and Micro-CT 3D reconstruction of femurs on day 28 post-treatment: white arrows (fracture location), red arrows (early callus), blue dotted lines (femur boundary), and yellow dashed lines (callus boundary). Scale bar of x-ray: 5 mm; Scale bar of 3D reconstruction: 1 mm. c. Quantitative analysis of the fracture callus BMD and BV/TV (normal PBS Ctrl group data from PBS group in d–g) (n = 6 per group). d. Representative H&E staining images and representative Masson's trichrome staining images of fracture calluses at 28 days. Scale bar: 50 μm. e. Quantification of the callus area/total bone area ratio and quantification of the new bone area/total bone area ratio (n = 6 per group). f, g. Representative IFHC images of the callus region at 28 days and quantification of the relative fluorescence intensities: (f) The white dotted line represents the boundary between the callus and muscles (M: muscle, C: callus); Runx2 (red), ALP (green), and DAPI (blue). Scale bar: 50 μm; (g) Quantification of the relative fluorescence intensities of Runx2 and ALP (n = 6 per group). h, i. Representative IFHC images of the callus region at 28 days and quantification of the relative fluorescence intensities: (h) The white dotted line represents the boundary between the callus and muscles (M: muscle, C: callus); <t>Sp7</t> (red), ALP (green), and DAPI (blue). Scale bar: 50 μm; (i) Quantification of the relative fluorescence intensities of Sp7 and ALP (n = 6 per group). The data are presented as the means ± standard deviations (SDs). Unpaired Student's t -test was used for two-group comparisons. One-way ANOVA was used for multiple comparisons. Significance levels: ∗ p < 0.05, ∗∗∗∗ p < 0.0001.
Cd3ε, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti osterix  (R&D Systems)
94
R&D Systems mouse anti osterix
D-Bmp2@M accelerates fracture healing in osteoporotic mice. a. Schematic of the osteoporotic fracture treatment procedure: C57BL/6 mice underwent bilateral ovariectomy (OVX) to establish an osteoporosis model, followed by transverse femoral fracture induction and 28 days of treatment. b. Representative X-ray images of the fracture healing process at different time points and Micro-CT 3D reconstruction of femurs on day 28 post-treatment: white arrows (fracture location), red arrows (early callus), blue dotted lines (femur boundary), and yellow dashed lines (callus boundary). Scale bar of x-ray: 5 mm; Scale bar of 3D reconstruction: 1 mm. c. Quantitative analysis of the fracture callus BMD and BV/TV (normal PBS Ctrl group data from PBS group in d–g) (n = 6 per group). d. Representative H&E staining images and representative Masson's trichrome staining images of fracture calluses at 28 days. Scale bar: 50 μm. e. Quantification of the callus area/total bone area ratio and quantification of the new bone area/total bone area ratio (n = 6 per group). f, g. Representative IFHC images of the callus region at 28 days and quantification of the relative fluorescence intensities: (f) The white dotted line represents the boundary between the callus and muscles (M: muscle, C: callus); Runx2 (red), ALP (green), and DAPI (blue). Scale bar: 50 μm; (g) Quantification of the relative fluorescence intensities of Runx2 and ALP (n = 6 per group). h, i. Representative IFHC images of the callus region at 28 days and quantification of the relative fluorescence intensities: (h) The white dotted line represents the boundary between the callus and muscles (M: muscle, C: callus); <t>Sp7</t> (red), ALP (green), and DAPI (blue). Scale bar: 50 μm; (i) Quantification of the relative fluorescence intensities of Sp7 and ALP (n = 6 per group). The data are presented as the means ± standard deviations (SDs). Unpaired Student's t -test was used for two-group comparisons. One-way ANOVA was used for multiple comparisons. Significance levels: ∗ p < 0.05, ∗∗∗∗ p < 0.0001.
Mouse Anti Osterix, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd3  (Novus Biologicals)
95
Novus Biologicals anti cd3
D-Bmp2@M accelerates fracture healing in osteoporotic mice. a. Schematic of the osteoporotic fracture treatment procedure: C57BL/6 mice underwent bilateral ovariectomy (OVX) to establish an osteoporosis model, followed by transverse femoral fracture induction and 28 days of treatment. b. Representative X-ray images of the fracture healing process at different time points and Micro-CT 3D reconstruction of femurs on day 28 post-treatment: white arrows (fracture location), red arrows (early callus), blue dotted lines (femur boundary), and yellow dashed lines (callus boundary). Scale bar of x-ray: 5 mm; Scale bar of 3D reconstruction: 1 mm. c. Quantitative analysis of the fracture callus BMD and BV/TV (normal PBS Ctrl group data from PBS group in d–g) (n = 6 per group). d. Representative H&E staining images and representative Masson's trichrome staining images of fracture calluses at 28 days. Scale bar: 50 μm. e. Quantification of the callus area/total bone area ratio and quantification of the new bone area/total bone area ratio (n = 6 per group). f, g. Representative IFHC images of the callus region at 28 days and quantification of the relative fluorescence intensities: (f) The white dotted line represents the boundary between the callus and muscles (M: muscle, C: callus); Runx2 (red), ALP (green), and DAPI (blue). Scale bar: 50 μm; (g) Quantification of the relative fluorescence intensities of Runx2 and ALP (n = 6 per group). h, i. Representative IFHC images of the callus region at 28 days and quantification of the relative fluorescence intensities: (h) The white dotted line represents the boundary between the callus and muscles (M: muscle, C: callus); <t>Sp7</t> (red), ALP (green), and DAPI (blue). Scale bar: 50 μm; (i) Quantification of the relative fluorescence intensities of Sp7 and ALP (n = 6 per group). The data are presented as the means ± standard deviations (SDs). Unpaired Student's t -test was used for two-group comparisons. One-way ANOVA was used for multiple comparisons. Significance levels: ∗ p < 0.05, ∗∗∗∗ p < 0.0001.
Anti Cd3, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd3  (Novus Biologicals)
95
Novus Biologicals cd3
D-Bmp2@M accelerates fracture healing in osteoporotic mice. a. Schematic of the osteoporotic fracture treatment procedure: C57BL/6 mice underwent bilateral ovariectomy (OVX) to establish an osteoporosis model, followed by transverse femoral fracture induction and 28 days of treatment. b. Representative X-ray images of the fracture healing process at different time points and Micro-CT 3D reconstruction of femurs on day 28 post-treatment: white arrows (fracture location), red arrows (early callus), blue dotted lines (femur boundary), and yellow dashed lines (callus boundary). Scale bar of x-ray: 5 mm; Scale bar of 3D reconstruction: 1 mm. c. Quantitative analysis of the fracture callus BMD and BV/TV (normal PBS Ctrl group data from PBS group in d–g) (n = 6 per group). d. Representative H&E staining images and representative Masson's trichrome staining images of fracture calluses at 28 days. Scale bar: 50 μm. e. Quantification of the callus area/total bone area ratio and quantification of the new bone area/total bone area ratio (n = 6 per group). f, g. Representative IFHC images of the callus region at 28 days and quantification of the relative fluorescence intensities: (f) The white dotted line represents the boundary between the callus and muscles (M: muscle, C: callus); Runx2 (red), ALP (green), and DAPI (blue). Scale bar: 50 μm; (g) Quantification of the relative fluorescence intensities of Runx2 and ALP (n = 6 per group). h, i. Representative IFHC images of the callus region at 28 days and quantification of the relative fluorescence intensities: (h) The white dotted line represents the boundary between the callus and muscles (M: muscle, C: callus); <t>Sp7</t> (red), ALP (green), and DAPI (blue). Scale bar: 50 μm; (i) Quantification of the relative fluorescence intensities of Sp7 and ALP (n = 6 per group). The data are presented as the means ± standard deviations (SDs). Unpaired Student's t -test was used for two-group comparisons. One-way ANOVA was used for multiple comparisons. Significance levels: ∗ p < 0.05, ∗∗∗∗ p < 0.0001.
Cd3, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary antibodies against cd3  (Novus Biologicals)
95
Novus Biologicals primary antibodies against cd3
(A) Population restricted FC analysis to identify CD45 + Ly6G − NK1.1 − CD19 − <t>CD3</t> + T cells in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of T cells calculated as a percentage of total CD45 + cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of T cells per uterine horn. (D) Immunohistochemistry: detection of CD3 + T cells (black arrows) in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=5, 48 hr n=6, scale bar= 50µm). (E) Population restricted FC analysis to identify CD45 + Ly6G − CD3 − CD19 − NK1.1 + NK cells in uterine tissue digests. (F) Bar plot, FC quantification: Relative abundance of NK cells calculated as a percentage of total CD45 + cells per uterine horn. (G) Bar plot, FC quantification: Absolute counts of NK cells per uterine horn. (H) Immunohistochemistry: detection of DBA-bound NK cells in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=4, 48 hr n=4, scale bar= 50µm). (I) Population restricted FC analysis to identify CD45 + Ly6G − CD3 − NK1.1 − CD19 + B cells in uterine tissue digests (J) Bar plot, FC quantification: Relative abundance of B cells calculated as a percentage of total CD45 + cells per uterine horn. (K) Bar plot, FC quantification: Absolute counts of B cells per uterine horn. (L) Immunohistochemistry: detection of B220 + B cells (black arrows) in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=4, 48 hr n=4, scale bar= 50µm). (FC analysis groups: control (n=15), 0 hr (prior to breakdown, n=17), 12 hr (tissue breakdown, n=22), 24 hr (tissue repair, n=16) and 48 hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
Primary Antibodies Against Cd3, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti sp7 osterix  (R&D Systems)
94
R&D Systems anti sp7 osterix
(A) Population restricted FC analysis to identify CD45 + Ly6G − NK1.1 − CD19 − <t>CD3</t> + T cells in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of T cells calculated as a percentage of total CD45 + cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of T cells per uterine horn. (D) Immunohistochemistry: detection of CD3 + T cells (black arrows) in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=5, 48 hr n=6, scale bar= 50µm). (E) Population restricted FC analysis to identify CD45 + Ly6G − CD3 − CD19 − NK1.1 + NK cells in uterine tissue digests. (F) Bar plot, FC quantification: Relative abundance of NK cells calculated as a percentage of total CD45 + cells per uterine horn. (G) Bar plot, FC quantification: Absolute counts of NK cells per uterine horn. (H) Immunohistochemistry: detection of DBA-bound NK cells in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=4, 48 hr n=4, scale bar= 50µm). (I) Population restricted FC analysis to identify CD45 + Ly6G − CD3 − NK1.1 − CD19 + B cells in uterine tissue digests (J) Bar plot, FC quantification: Relative abundance of B cells calculated as a percentage of total CD45 + cells per uterine horn. (K) Bar plot, FC quantification: Absolute counts of B cells per uterine horn. (L) Immunohistochemistry: detection of B220 + B cells (black arrows) in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=4, 48 hr n=4, scale bar= 50µm). (FC analysis groups: control (n=15), 0 hr (prior to breakdown, n=17), 12 hr (tissue breakdown, n=22), 24 hr (tissue repair, n=16) and 48 hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
Anti Sp7 Osterix, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


D-Bmp2@M accelerates fracture healing in osteoporotic mice. a. Schematic of the osteoporotic fracture treatment procedure: C57BL/6 mice underwent bilateral ovariectomy (OVX) to establish an osteoporosis model, followed by transverse femoral fracture induction and 28 days of treatment. b. Representative X-ray images of the fracture healing process at different time points and Micro-CT 3D reconstruction of femurs on day 28 post-treatment: white arrows (fracture location), red arrows (early callus), blue dotted lines (femur boundary), and yellow dashed lines (callus boundary). Scale bar of x-ray: 5 mm; Scale bar of 3D reconstruction: 1 mm. c. Quantitative analysis of the fracture callus BMD and BV/TV (normal PBS Ctrl group data from PBS group in d–g) (n = 6 per group). d. Representative H&E staining images and representative Masson's trichrome staining images of fracture calluses at 28 days. Scale bar: 50 μm. e. Quantification of the callus area/total bone area ratio and quantification of the new bone area/total bone area ratio (n = 6 per group). f, g. Representative IFHC images of the callus region at 28 days and quantification of the relative fluorescence intensities: (f) The white dotted line represents the boundary between the callus and muscles (M: muscle, C: callus); Runx2 (red), ALP (green), and DAPI (blue). Scale bar: 50 μm; (g) Quantification of the relative fluorescence intensities of Runx2 and ALP (n = 6 per group). h, i. Representative IFHC images of the callus region at 28 days and quantification of the relative fluorescence intensities: (h) The white dotted line represents the boundary between the callus and muscles (M: muscle, C: callus); Sp7 (red), ALP (green), and DAPI (blue). Scale bar: 50 μm; (i) Quantification of the relative fluorescence intensities of Sp7 and ALP (n = 6 per group). The data are presented as the means ± standard deviations (SDs). Unpaired Student's t -test was used for two-group comparisons. One-way ANOVA was used for multiple comparisons. Significance levels: ∗ p < 0.05, ∗∗∗∗ p < 0.0001.

Journal: Bioactive Materials

Article Title: Sustained release PLGA microspheres loaded with a bone-affinity Bmp2 enhance fracture healing and mitigate heterotopic ossification

doi: 10.1016/j.bioactmat.2026.02.050

Figure Lengend Snippet: D-Bmp2@M accelerates fracture healing in osteoporotic mice. a. Schematic of the osteoporotic fracture treatment procedure: C57BL/6 mice underwent bilateral ovariectomy (OVX) to establish an osteoporosis model, followed by transverse femoral fracture induction and 28 days of treatment. b. Representative X-ray images of the fracture healing process at different time points and Micro-CT 3D reconstruction of femurs on day 28 post-treatment: white arrows (fracture location), red arrows (early callus), blue dotted lines (femur boundary), and yellow dashed lines (callus boundary). Scale bar of x-ray: 5 mm; Scale bar of 3D reconstruction: 1 mm. c. Quantitative analysis of the fracture callus BMD and BV/TV (normal PBS Ctrl group data from PBS group in d–g) (n = 6 per group). d. Representative H&E staining images and representative Masson's trichrome staining images of fracture calluses at 28 days. Scale bar: 50 μm. e. Quantification of the callus area/total bone area ratio and quantification of the new bone area/total bone area ratio (n = 6 per group). f, g. Representative IFHC images of the callus region at 28 days and quantification of the relative fluorescence intensities: (f) The white dotted line represents the boundary between the callus and muscles (M: muscle, C: callus); Runx2 (red), ALP (green), and DAPI (blue). Scale bar: 50 μm; (g) Quantification of the relative fluorescence intensities of Runx2 and ALP (n = 6 per group). h, i. Representative IFHC images of the callus region at 28 days and quantification of the relative fluorescence intensities: (h) The white dotted line represents the boundary between the callus and muscles (M: muscle, C: callus); Sp7 (red), ALP (green), and DAPI (blue). Scale bar: 50 μm; (i) Quantification of the relative fluorescence intensities of Sp7 and ALP (n = 6 per group). The data are presented as the means ± standard deviations (SDs). Unpaired Student's t -test was used for two-group comparisons. One-way ANOVA was used for multiple comparisons. Significance levels: ∗ p < 0.05, ∗∗∗∗ p < 0.0001.

Article Snippet: The sections were then incubated at 4 °C for 1 h with 5% bovine serum albumin and subsequently incubated overnight with anti-Runx2 antibody (1:300, Beyotime, China), anti-Sp7 antibody (1:300, MCE, China), and anti-Flag antibody (1:200, Servicebio).

Techniques: Micro-CT, Staining, Fluorescence, Muscles

(A) Population restricted FC analysis to identify CD45 + Ly6G − NK1.1 − CD19 − CD3 + T cells in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of T cells calculated as a percentage of total CD45 + cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of T cells per uterine horn. (D) Immunohistochemistry: detection of CD3 + T cells (black arrows) in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=5, 48 hr n=6, scale bar= 50µm). (E) Population restricted FC analysis to identify CD45 + Ly6G − CD3 − CD19 − NK1.1 + NK cells in uterine tissue digests. (F) Bar plot, FC quantification: Relative abundance of NK cells calculated as a percentage of total CD45 + cells per uterine horn. (G) Bar plot, FC quantification: Absolute counts of NK cells per uterine horn. (H) Immunohistochemistry: detection of DBA-bound NK cells in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=4, 48 hr n=4, scale bar= 50µm). (I) Population restricted FC analysis to identify CD45 + Ly6G − CD3 − NK1.1 − CD19 + B cells in uterine tissue digests (J) Bar plot, FC quantification: Relative abundance of B cells calculated as a percentage of total CD45 + cells per uterine horn. (K) Bar plot, FC quantification: Absolute counts of B cells per uterine horn. (L) Immunohistochemistry: detection of B220 + B cells (black arrows) in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=4, 48 hr n=4, scale bar= 50µm). (FC analysis groups: control (n=15), 0 hr (prior to breakdown, n=17), 12 hr (tissue breakdown, n=22), 24 hr (tissue repair, n=16) and 48 hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Journal: bioRxiv

Article Title: Multimodal Profiling of Repair-associated Immune Dynamics in a Mouse Model of Menstruation

doi: 10.64898/2026.02.06.704418

Figure Lengend Snippet: (A) Population restricted FC analysis to identify CD45 + Ly6G − NK1.1 − CD19 − CD3 + T cells in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of T cells calculated as a percentage of total CD45 + cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of T cells per uterine horn. (D) Immunohistochemistry: detection of CD3 + T cells (black arrows) in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=5, 48 hr n=6, scale bar= 50µm). (E) Population restricted FC analysis to identify CD45 + Ly6G − CD3 − CD19 − NK1.1 + NK cells in uterine tissue digests. (F) Bar plot, FC quantification: Relative abundance of NK cells calculated as a percentage of total CD45 + cells per uterine horn. (G) Bar plot, FC quantification: Absolute counts of NK cells per uterine horn. (H) Immunohistochemistry: detection of DBA-bound NK cells in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=4, 48 hr n=4, scale bar= 50µm). (I) Population restricted FC analysis to identify CD45 + Ly6G − CD3 − NK1.1 − CD19 + B cells in uterine tissue digests (J) Bar plot, FC quantification: Relative abundance of B cells calculated as a percentage of total CD45 + cells per uterine horn. (K) Bar plot, FC quantification: Absolute counts of B cells per uterine horn. (L) Immunohistochemistry: detection of B220 + B cells (black arrows) in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=4, 48 hr n=4, scale bar= 50µm). (FC analysis groups: control (n=15), 0 hr (prior to breakdown, n=17), 12 hr (tissue breakdown, n=22), 24 hr (tissue repair, n=16) and 48 hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Article Snippet: Primary antibodies against CD3 (1:250 dilution; NB600-1441, Novus Biologicals), B220 (1:250 dilution; 14-0452-82, Invitrogen), CD14 (1:6000 dilution; ab221678, Abcam), CD64 (1:6000 dilution; 50086-R008, Sino Biological), Ly6G (1:10000 dilution; 127649, Biolegend) and DBA-lectin (1:1000 dilution; B-1035, Vector) were used in this study.

Techniques: Immunohistochemistry, Control

Immunohistochemistry: detection of (A) CD3+ T cells, (B) DBA-lectin+ NK cells and (C) B220+ B cells in uterine tissue sections at 0hr (prior to breakdown, n=4), 12hr (tissue breakdown, n=4-6), 24hr (tissue repair, n=4-5) and 48hr (tissue remodelling, n=4-6) hr after progesterone withdrawal (representative images, scale bar=500µm).

Journal: bioRxiv

Article Title: Multimodal Profiling of Repair-associated Immune Dynamics in a Mouse Model of Menstruation

doi: 10.64898/2026.02.06.704418

Figure Lengend Snippet: Immunohistochemistry: detection of (A) CD3+ T cells, (B) DBA-lectin+ NK cells and (C) B220+ B cells in uterine tissue sections at 0hr (prior to breakdown, n=4), 12hr (tissue breakdown, n=4-6), 24hr (tissue repair, n=4-5) and 48hr (tissue remodelling, n=4-6) hr after progesterone withdrawal (representative images, scale bar=500µm).

Article Snippet: Primary antibodies against CD3 (1:250 dilution; NB600-1441, Novus Biologicals), B220 (1:250 dilution; 14-0452-82, Invitrogen), CD14 (1:6000 dilution; ab221678, Abcam), CD64 (1:6000 dilution; 50086-R008, Sino Biological), Ly6G (1:10000 dilution; 127649, Biolegend) and DBA-lectin (1:1000 dilution; B-1035, Vector) were used in this study.

Techniques: Immunohistochemistry

(A) Population restricted FC analysis to identify CD45 + CD3 − CD19 − NK1.1 − CD11b + Ly6G − SiglecF − CD64 − Ly6C + MHCII − monocytes in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of monocytes calculated as a percentage of total CD45 + cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of monocytes per uterine horn. (D) Population restricted FC analysis of CD45 + CD3 − CD19 − NK1.1 − CD11b + Ly6G − SiglecF − CD64 + Ly6C − F4/80 + MHCII + macrophages in uterine tissue digests. (E) Bar plot, FC quantification: Relative abundance of macrophages calculated as a percentage of total CD45 + cells per uterine horn. (F) Bar plot, FC quantification: Absolute counts of macrophages per uterine horn. (G) Population restricted FC analysis of CD45 + CD3 − CD19 − NK1.1 − CD11b + Ly6G + SiglecF − neutrophils in uterine tissue digests. (H) Bar plot, FC quantification: Relative abundance of neutrophils calculated as a percentage of total CD45 + cells per uterine horn. (I) Bar plot, FC quantification: Absolute counts of neutrophils per uterine horn. (FC analysis groups: control (n=15), 0 hr (prior to breakdown, n=17), 12 hr (tissue breakdown, n=22), 24 hr (tissue repair, n=16) and 48 hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). (J-K) Immunohistochemical detection of CD14 + monocytes (cyan), CD64 + macrophages (yellow) and Ly6G + neutrophils (magenta) (merged and split channel) in uterine tissue cross sections at (J) 24 hr (repair, n=5) and (K) 48 hr (remodelling, n=3) following progesterone withdrawal (merged and split channel representative images, scale bar = 1000µm).

Journal: bioRxiv

Article Title: Multimodal Profiling of Repair-associated Immune Dynamics in a Mouse Model of Menstruation

doi: 10.64898/2026.02.06.704418

Figure Lengend Snippet: (A) Population restricted FC analysis to identify CD45 + CD3 − CD19 − NK1.1 − CD11b + Ly6G − SiglecF − CD64 − Ly6C + MHCII − monocytes in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of monocytes calculated as a percentage of total CD45 + cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of monocytes per uterine horn. (D) Population restricted FC analysis of CD45 + CD3 − CD19 − NK1.1 − CD11b + Ly6G − SiglecF − CD64 + Ly6C − F4/80 + MHCII + macrophages in uterine tissue digests. (E) Bar plot, FC quantification: Relative abundance of macrophages calculated as a percentage of total CD45 + cells per uterine horn. (F) Bar plot, FC quantification: Absolute counts of macrophages per uterine horn. (G) Population restricted FC analysis of CD45 + CD3 − CD19 − NK1.1 − CD11b + Ly6G + SiglecF − neutrophils in uterine tissue digests. (H) Bar plot, FC quantification: Relative abundance of neutrophils calculated as a percentage of total CD45 + cells per uterine horn. (I) Bar plot, FC quantification: Absolute counts of neutrophils per uterine horn. (FC analysis groups: control (n=15), 0 hr (prior to breakdown, n=17), 12 hr (tissue breakdown, n=22), 24 hr (tissue repair, n=16) and 48 hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). (J-K) Immunohistochemical detection of CD14 + monocytes (cyan), CD64 + macrophages (yellow) and Ly6G + neutrophils (magenta) (merged and split channel) in uterine tissue cross sections at (J) 24 hr (repair, n=5) and (K) 48 hr (remodelling, n=3) following progesterone withdrawal (merged and split channel representative images, scale bar = 1000µm).

Article Snippet: Primary antibodies against CD3 (1:250 dilution; NB600-1441, Novus Biologicals), B220 (1:250 dilution; 14-0452-82, Invitrogen), CD14 (1:6000 dilution; ab221678, Abcam), CD64 (1:6000 dilution; 50086-R008, Sino Biological), Ly6G (1:10000 dilution; 127649, Biolegend) and DBA-lectin (1:1000 dilution; B-1035, Vector) were used in this study.

Techniques: Control, Immunohistochemical staining

(A) Population restricted FC analysis to identify CD45+ CD3- CD19- NK1.1- Ly6G- CD64- CD11c+ MHCII+ dendritic cells (DCs) in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of DCs calculated as a percentage of total CD45+ cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of DCs per uterine horn. (D) Population restricted FC analysis to CD45+ CD3- CD19- NK1.1- CD11b+ Ly6G- SiglecF+ eosinophils in uterine tissue digests. (E) Bar plot, FC quantification: Relative abundance of eosinophils calculated as a percentage of total CD45+ cells per uterine horn. (F) Bar plot, FC quantification: Absolute counts of eosinophils per uterine horn. (FC analysis groups: control (n=15), 0hr (prior to breakdown, n=17), 12hr (tissue breakdown, n=22), 24hr (tissue repair, n=16) and 48hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. and * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Journal: bioRxiv

Article Title: Multimodal Profiling of Repair-associated Immune Dynamics in a Mouse Model of Menstruation

doi: 10.64898/2026.02.06.704418

Figure Lengend Snippet: (A) Population restricted FC analysis to identify CD45+ CD3- CD19- NK1.1- Ly6G- CD64- CD11c+ MHCII+ dendritic cells (DCs) in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of DCs calculated as a percentage of total CD45+ cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of DCs per uterine horn. (D) Population restricted FC analysis to CD45+ CD3- CD19- NK1.1- CD11b+ Ly6G- SiglecF+ eosinophils in uterine tissue digests. (E) Bar plot, FC quantification: Relative abundance of eosinophils calculated as a percentage of total CD45+ cells per uterine horn. (F) Bar plot, FC quantification: Absolute counts of eosinophils per uterine horn. (FC analysis groups: control (n=15), 0hr (prior to breakdown, n=17), 12hr (tissue breakdown, n=22), 24hr (tissue repair, n=16) and 48hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. and * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Article Snippet: Primary antibodies against CD3 (1:250 dilution; NB600-1441, Novus Biologicals), B220 (1:250 dilution; 14-0452-82, Invitrogen), CD14 (1:6000 dilution; ab221678, Abcam), CD64 (1:6000 dilution; 50086-R008, Sino Biological), Ly6G (1:10000 dilution; 127649, Biolegend) and DBA-lectin (1:1000 dilution; B-1035, Vector) were used in this study.

Techniques: Control